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詳細介紹
| 品牌 | absin | 供貨周期 | 兩周 |
|---|
| 概述 | |
|---|---|
| 描述 | Purified Mouse Monoclonal Antibody (Mab) |
| 別名 | Histone H31, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA |
| 克隆類別 | Monoclonal |
| 克隆號 | 809CT10.4.2 |
| 同種亞型 | IgG1 |
| 反應種屬
| H, R, M |
預測反應種屬  | Bovine |
| 來源宿主 | Mouse |
| 應用 | WB |
| 形態 | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| 使用方法 | WB~~1:2000 |
| 性能 | |
|---|---|
| 生產說明 | This Histone H3 antibody is generated from a mouse immunized with Histone H3 recombinant protein. |
| 抗原來源 | HUMAN |
| 背景 | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| 保存方法 | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| 研究領域 | Crown Antibodies |
| 分子量 | 15.4 kDa |
| 參考文獻 | |
|---|---|
| Zhong R.,et al.Nucleic Acids Res. 11:7409-7425(1983). Marashi F.,et al.Biochem. Cell Biol. 64:277-289(1986). Albig W.,et al.Genomics 10:940-948(1991). Kardalinou E.,et al.J. Cell. Biochem. 52:375-383(1993). Runge D.,et al.Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases. | |
實驗結果圖
Western blot analysis of lysates from Hela, MCF-7, C6 cell line (from left to right), using Histone Antibody (H3)(Cat. #abs100462). abs100462 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
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