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簡(jiǎn)要描述：雞胚提取物是從生長(zhǎng)9-12天的雞胚中提取的，用于組織培養(yǎng)中的生長(zhǎng)因子補(bǔ)充成分。制備經(jīng)無(wú)菌過(guò)濾（0.2μm），以10毫升凍干小瓶的形式提供。請(qǐng)注意，凍干產(chǎn)品無(wú)法保證無(wú)菌，重構(gòu)后應(yīng)通過(guò)無(wú)菌一次性過(guò)濾器重新過(guò)濾。
產(chǎn)品型號(hào)：abs80002廠商性質(zhì)：生產(chǎn)廠家更新時(shí)間：2025-10-29訪 問 量：2751產(chǎn)品分類
Product Category詳細(xì)介紹
| 品牌 | absin | 貨號(hào) | abs80002 |
|---|---|---|---|
| 規(guī)格 | 10mL | 供貨周期 | 現(xiàn)貨 |
| 主要用途 | 用于組織培養(yǎng)中的生長(zhǎng)因子補(bǔ)充成分。 | 應(yīng)用領(lǐng)域 | 化工,生物產(chǎn)業(yè),農(nóng)林牧漁,制藥/生物制藥,綜合 |
| 產(chǎn)品描述 | |
| 描述 | 雞胚提取物是從生長(zhǎng)9-12天的雞胚中提取的，用于組織培養(yǎng)中的生長(zhǎng)因子補(bǔ)充成分。 The proprietary process utilises 9-12 day eggs collected from registered flocks and found to be free from clinical signs of notifiable diseases. It is processed as a 50% w/v extract in Dulbecco's Phosphate Buffered Saline. 100IU/ml Penicillin，100μg/ml Streptomycin and 2.5μg/ml Amphotericin are added as an additional safeguard. Statistical samples representative of the batch are tested in agreement with cGLP to validate the product as being consistent with the designed quality standards of reliability and stability. |
| 應(yīng)用 | The product is intended for in-vitro cell culture only. |
| 儲(chǔ)存/保存方法 | Recommended storage is -20°C or below. Lyophilized product may be stored at 2-8°C. |
| 產(chǎn)品用途 | Directions for Use 1、Thaw the product overnight at 4°C and then bring to room temperature. 2、Use a 0.2um filtered ultra-purified water, and add to vials using a 10mL syringe with a hypodermic needle. Allow water to be draw in by vacuum. 3、Place samples on their sides and mix using an orbital shaker set at 150rpm at room temperature for 30 minutes. 4、To filter, use sterile gauze if necessary, then a 0.45um filter, then finally use a 0.2um filter. |
| 注意事項(xiàng) | 用10毫升無(wú)菌組織培養(yǎng)級(jí)水重新配制凍干產(chǎn)品。攪拌混合。如果出現(xiàn)沉淀物，在冷卻條件下繼續(xù)攪拌數(shù)小時(shí)/一夜或超聲處理，簡(jiǎn)要使用超聲波水浴。然后，在3000 x g下離心10分鐘，并移除顆粒。之后重組凍干產(chǎn)品必須通過(guò)0.2µm過(guò)濾器過(guò)濾，以保持無(wú)菌。復(fù)原后，在4°C下儲(chǔ)存以供立即使用，或等份加入工作體積，并在-20至-10°C長(zhǎng)期儲(chǔ)存，最大限度地減少凍融循環(huán)解凍時(shí)可觀察到蛋白質(zhì)沉淀。為了消除這種情況，可以在3000×g的條件下離心10分鐘。 |
| 基本信息 | |
| 別名 | 雞胚提取液;CEE；Chicken Embryo Extract |
| 可溶性/溶解性 | Reconstitute with water to 50% w/v. Solutions can be filtered through a 0.2 μm sterile filter as sterile. |
| 參考文獻(xiàn) | |
| 參考文獻(xiàn) | 1. Yablonka-Reuveni, Z., "Myogenesis in the chicken: The onset of differentiation of adultmyoblasts is influenced by tissue factors." Basic and Applied Myology, v. 5(1), 33 (1995). 2. Beurg, M., et al., "Differential regulation of skeletal muscle L-Type Ca2+ current and excitation -contraction coupling by the dihydropyridine receptor beta subunit." BiophysicalJournal, v. 76, 1744-1756 (1999). |
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